Gelprint is a tool that generates an in silico 2D-gel from the theoretical pI (isoelectric point) and Mw (molecular weight) data for a set of proteins. Comparisons of the in silico 2D-gel with your real 2D-gel generated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) may help you to identify proteins of your interest.

The in silico 2D-gel in printable formats (postscript and PDF) is scaled to fit to your real gel. You can physically overlay and compare the two different (in silico and experiemntal) gels by printing them on transparenceis. Gelprint also outputs a clickable in silico 2D-gel.

Click links here to see example pages for R. conorii, R. prowazekii and T. whipplei filled with sample data.


1. Enter a list of theoretical pI and Mw for proteins (sample):

2. Enter the physical size of your gel in centi meter: 3. Enter the area of gel to print:
Gelprint scales either or both axes (pI and Mw) to obtain better surperpositions between the in silico gel and the real gel. If you wish this function, please specify several reference points using the following sections 3 and 4. Given no reference points, Gelprint adjusts the printed area (specified in section 3) to the physical size of the gel (specified in section 2).

4. Enter the reference points for pI (sample):

5. Enter the reference points for Mw (sample):


Additional options can be specified below.

6. Specify the database link if appropriate:

7. Scaling for postscript and PDF if necessary:

A number of useful proteomics tools, including the calculation of theoretical pI and Mw, are aveilable at ExPASy Proteomics tools.
We appreciate your feedback regarding Gelprint to Hiroyuki.Ogata@igs.cnrs-mrs.fr.