Metagrowth uses the data of the component concentrations in known culture media to provide appropriate ranges of nutriment concentrations when users intend to design new culture media.
Currently we use the data for the following complex culture media for fastidious parasitic bacteria: The components in these complex media can be classified into several categories according to their putative principal roles in bacterial growth. As shown in the figure below, the concentrations of the chemical components largely varies between different categories, but rather limited in a narrower range within a category (more detail can be seen in another Figure). The range for each category is used to suggest appropriate samplings of nutriment concentrations.

BSK-H complete medium

Components mg/L micro-M
Glycine 47 626
L-Alanine 23.5 264
L-Arginine 54.398 312.27
L-Aspartic Acid 28.2 212
L-Cysteine.HCl.H2O 244.4 1392
L-Cystine 18.8 78.2
L-Glutamic Acid 70.5 479
L-Histidine HCl.H2O 18.8 89.7
L-Isoleucine 18.8 143
L-Leucine 56.4 430
L-Lysine.HCl 65.8 360
L-Methionine 14.1 94.5
L-Phenylalanine 23.5 142
L-Proline 37.6 327
L-Serine 23.5 224
L-Threonine 28.2 237
L-Tryptophan 9.4 46
L-Tyrosine 37.6 208
L-Valine 23.5 201
2'-Deoxyadenosine 9.4 37
2'-Deoxycytidine.HCL 10.904 41.353
2'-Deoxyguanosine 9.4 35
5-Methyldeoxycytidine 0.094 0.39
Thymidine 9.4 39
Uridine-5-Triphosphate.Na 0.94 1.9
Citric Acid.3Na.2H2O 695.6 2341
D-Glucose 5640 31306
Pyruvic Acid.Na 752 6772
Trans-4-Hydroxy-L-Proline 9.4 72
N-Acetyl-D-Glucosamine 376 1700
Cholesterol 0.188 0.486
Choline Chloride 0.47 3.4
myo-Inositol 0.047 0.26
D-Glucuronic Acid.Na 3.65 16.8
beta-NAD 6.956 10.47
beta-NADP.Na 0.94 1.4
Cocarboxylase (i.e.Thiamine Diphosphate) 0.94 2.2
Coenzyme A 2.35 3.06
D-Biotin 0.0094 0.038
D-Pantothenic Acid.Hemicalcium 0.0094 0.039
Flavin Adenine Dinucleotide.2Na 0.0996 0.121
Folic Acid 0.0094 0.021
Glutathione 9.4 31
Niacinamide (i.e. Nicotinamide) 0.0235 0.192
Nicotinic Acid 0.0235 0.191
PABA 0.047 0.34
Pyridoxal.HCL 0.0235 0.115
Pyridoxine.HCL 0.0235 0.114
Riboflavin 0.0094 0.025
Thiamine.HCL 0.0094 0.031
Calcium Chloride [Anhydrous] 188 1694
Magnesium Sulfate [Anhydrous] 91.83 762.9
Potassium Chloride 376 5044
Sodium Acetate [Anhydrous] 47 573
Sodium Bicarbonate 2068 24620
Sodium Chloride 6392 109400
Sodium Phosphate Monobasic [Anhydrous] 114.68 955.85
Albumin, Bovine 47000 -
HEPES (i.e. 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 5640 23670
L-Ascorbic Acid 47 270
Neopeptone 4700 -
Phenol Red.Na 19.97 53.06
Rabbit Serum 60ml/l -
Tween 80 (i.e. Polysorbate 80) 4.7 3.5
Yeast Extract 1880 -

Tropheryma whipplei medium (TWH)

Components mg/L micro-M
Glycine 18.75 249.8
L-Alanine 4.45 49.95
L-Arginine.HCl 147.5 700.2
L-Asparagine.H2O 7.5 51.0
L-Aspartate 6.65 50.0
L-Cystine HCl.H2O 17.56 59.57
L-Cystine.2HCl 31.29 100.0
L-Glutamate 7.35 50.0
L-Glutamine 365 2497
L-Histidine HCl.H2O 31.48 150.2
L-Isoleucine 54.47 415.2
L-Leucine 59.05 450.2
L-Lysine HCl 91.25 499.6
L-Methionine 17.24 115.5
L-Phenylalanine 35.48 214.8
L-Proline 17.25 149.8
L-Serine 26.25 249.8
L-Threonine 53.45 448.7
L-Tryptophane 9.02 44.2
L-Tyrosine.2Na.2H2O 55.79 212.0
L-Valine 25.85 220.7
Hypoxanthine 2.39 17.6
Thymidine 0.365 1.51
D-Glucose 3151 17490
Sodium Pyruvate 55 495
Choline Chloride 8.98 64.3
i-Inositol (i.e. myo-inositol) 12.6 70.0
Linoleic acid 0.042 0.15
Biotine 0.0035 0.014
D-Ca Pantothenate 2.24 8.64
Folate 2.65 6.00
Lipoic acid (Thioctic acid (DL-68?)) 0.105 0.509
Nicotinamide 2.02 16.5
Pyridoxine HCl 2.031 9.876
Riboflavine 0.219 0.5812
Sodium Putrescine.2HCl 0.081 0.44
Thiamine HCl 2.17 7.19
Vitamine B12 (Cobamide coenzyme) 0.68 0.43
CaCl2 (anhyd) 116.6 1051
CuSO4.5H2O 0.0013 0.0052
Fe(NO3)3.9H2O 0.05 0.12
FeSO4.7H2O 0.417 1.50
KCl 311.8 4182
MgCl2.6H2O 61 300
MgSO4.7H2O 100 406
Na2HPO4.7H2O 134 500
NaCl 6996 119700
NaH2PO4.H2O 62.5 453
NaHCO3 1200 14280
ZnSO4.7H2O 0.432 1.50
HEPES 3075.4 12905
Phenol red 8.1 23
Fetal calf serum 100ml/L -